Bacterial Reverse Mutation Test Evaluation of Hydrolyzed GMP Powder Containing Highly Concentrated Sialic Acid (23%) produced by Enzyme Separation and Solvent Enrichment Method

Mutagenicity of flavonoids assayed by bacterial reverse mutation (Ames) test.

The mutagenicity of ten flavonoids was assayed by the Ames test, in Salmonella typhimurium strains TA98, TA100 and TA102, with the aim of establishing hydroxylation pattern-mutagenicity relationship profiles. The compounds assessed were: quercetin, kaempferol, luteolin, fisetin, chrysin, galangin, flavone, 3-hydroxyflavone, 5-hydroxyflavone and 7-hydroxyflavone. In the Ames assay, quer...

enzyme digest and acid hydrolyzed index of protein quality evaluation

a pancreatopeptidase (elastase) digest index was devised for a rapid and accurate estimation of protein quality. this index was calculated on the basis of all the amino acids released by an in-vitro elastase digestion, acid hydrolyses of same sample and the residue of enzyme hydrolyzed. the amino acids were determined by thin-layer chromatography. samples used were cooked white kidneybeans, coo...

Protein separation and enrichment by counter-current chromatography using reverse micelle solvent systems.

A protein mixture consisting of myoglobin, cytochrome c, and lysozyme was separated by high-speed counter-current chromatography using a two-phase aqueous/reverse micelle-containing organic solvent system. About 50% stationary phase retention ratio was obtained in most chromatographic experiments. Separations were manipulated mainly by pH gradients that controlled the electrostatic interactions...

Safety Evaluation of Picrorhiza kurroa Rhizome Extract by Bacterial Reverse Mutation Test

Picrorhiza kurroa is a recognized herb in the Ayurvedic and Chinese system of traditional medicine. It is effective in treating a variety of diseases ranging from digestion problems to paralysis. Thus it is essential to rule out any kind of toxicity, mutagenicity or carcinogenicity in the plant to be used as a medicine. The study was conducted to evaluate ability of Picrorhiza kurroa to induce ...

Evaluation of the fabric formability by concentrated loading method